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KMID : 1007520000090050317
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Food Science and Biotechnology
2000 Volume.9 No. 5 p.317 ~ p.321
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Antioxidative activity of Ginkgo Biloba Leaves Derived Components on Free Radicals and Active Oxygen Species
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Kim, Young Soo
Lee, Hoi Seon/Kim, Moo Key/Lee, Sung Eun/Park, Byeoung Soo
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Abstract
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The antioxidative activity of Ginkgo biloba leaves was determined by measuring the inhibition of linoleic acid autoxidation and scavenging effects on DPPH radical and on superoxide anion. The Me₂C0 extract of G. biloba leaves showed strong antioxidative activity. The antioxidative activities of the fractionated extract were in the order of water>EtOAc>n-butanol fractions. All fractions exhibiting strong and moderate antioxidative activities were further purified via repeated silica gel, Amberlite IRN-78, Polyclar AT, Sephadex LH-20, and Sepralyte RP-18 column chromatographies. Antioxidant components were isolated and identified through ¹H-NMR and ^(13)C-NMR. The compounds were characterized as kaempferol 3-Ο-(2"-Ο-β-D-glucopyranosyl)-α-L-rhamnopyranoside, kaempferol 3-Ο-α-(6"´-p-coumaroyl-glucosyl-β-1,4-rhamnoside), and quercertin-3-Ο-α-(6"´-p-coumaroylglucosyl-β-1,4-rhammoside).
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